Synapsin I [p Ser603] Antibody - Azide Free

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - The figure depicts Western blots of the two synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6643/10/6/749) licensed under a CC-BY license.

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser603 in the first lane (-). Phosphospecificity is shown in thesecond lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (l-Ptase, 1200 units for 30 minutes).

Examples of Western blots of p-/synapsin 1 and p-/Ca2+-calmodulin-dependent kinase II. The figure depicts examples of the Western blots of the four synaptic plasticity markers investigated. The two bands seen in the p-synapsin and synapsin Western blots (70 and 74 kDa respectively) are consistent with splice variants as confirmed by manufacturer. P-CAMKII and CAMKII were detected around 50 kDa in accordance with previous findings.

Western Blot: Synapsin I [p Ser603] Antibody [NB300-181] - Examples of Western blots of p-/synapsin 1 & p-/Ca2+-calmodulin-dependent kinase II. The figure depicts examples of the Western blots of the four synaptic plasticity markers investigated. The two bands seen in the p-synapsin & synapsin Western blots (70 & 74 kDa respectively) are consistent with splice variants as confirmed by manufacturer. P-CAMKII & CAMKII were detected around 50 kDa in accordance with previous findings. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29890692), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Gp, Bv, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Synapsin I [p Ser603] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Synapsin I [p Ser603] Antibody - Azide Free (NB300-181) is a polyclonal antibody validated for use in WB. Anti-Synapsin I Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser603 conjugated to KLH. Accession # P17599
• Modification: p Ser603
• Localization: Cell junction; synapse
• Marker: pre-Synaptic Marker
• Specificity: Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser603 . Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Bovine (100%), Zebrafish (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Guinea Pig reactivity reported in (PMID: 29890692). Use in Human reported in secitific publication PMID: 32449987

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Synapsin I [p Ser603] Antibody - Azide Free

• phospho Synapsin
• SYN1
• SYN1a
• SYN1b
• Synapsin 1
• Synapsin I
• synapsin IBrain protein 4.1
• synapsin-1
• SYNI

Background

Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation. The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Synapsin I Antibody (NB300-181)(3)

Publications using NB300-181

• Castelli V, Melani F, Ferri C et al. Neuroprotective activities of bacopa, lycopene, astaxanthin, and vitamin B12 combination on oxidative stress-dependent neuronal death J. Cell. Biochem. 2020-05-25 [PMID: 32449987] (WB, Human)
• Hansen SN, Jorgensen JMB, NYengaard JR et al. Early Life Vitamin C Deficiency Does Not Alter Morphology of Hippocampal CA1 Pyramidal Neurons or Markers of Synaptic Plasticity in a Guinea Pig Model. Nutrients. 2018-06-08 [PMID: 29890692] (WB, Guinea Pig)
• Hansen SN, Ipsen DH, Schou-Pedersen AM et al. Long term Westernized diet leads to region-specific changes in brain signaling mechanisms. Neurosci. Lett. 2018-04-12 [PMID: 29655945] (Guinea Pig)

Bioinformatics

• Gene Symbol: SYN1
• Entrez:
  • 24949(Rat)