Synapsin I [p Ser9] Antibody - Azide Free

Western Blot: Synapsin I [p Ser9] Antibody [NB300-180] - Rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser9 synapsin I antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunohistochemistry: Synapsin I [p Ser9] Antibody [NB300-180] - Cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9

• Reactivity: Mu, Rt, Ze, Hu, Bv, Xp, Ze
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Synapsin I [p Ser9] Antibody - Azide Free Summary

• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH. Accession # P17599
• Modification: p Ser9
• Localization: Cell junction; synapse
• Marker: pre-Synaptic Marker
• Specificity: Specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser9. Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Xenopus (100%), Bovine (100%), Zebrafish (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SYN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500
  • Western Blot 1:1000
• Theoretical MW: 78 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Synapsin I [p Ser9] Antibody - Azide Free

• phospho Synapsin
• SYN1
• SYN1a
• SYN1b
• Synapsin 1
• Synapsin I
• synapsin IBrain protein 4.1
• synapsin-1
• SYNI

Background

Synapsin I (synapsin Ia and Ib, MW ~ 80,000 and 77,000 are collectively referred to as synapsin I) is a neuron specific protein that is localized to nerve terminals. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. It is thought that synapsin I cross-links synaptic vesicles to the cytoskeleton and thereby limits the ability of these synaptic vesicles to move to active zones and fuse with the plasma membrane during exocytosis. In addition to their role in neurotransmission, the synapsins are also thought to play a role in synapse formation. The appearance of synapsin I immunoreactivity correlates precisely with the development of synapses in the CNS. The synapsin protein is an excellent marker for synaptic terminals and it can be used to estimate synaptic density and or synaptogenesis. The appearance of synapsin I was a precise indicator of synapse formation and that synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYN1
• Entrez:
  • 20964(Mouse)
  • 24949(Rat)
• Uniprot:
  • P09951(Rat)