SYK Antibody (8C1A3)

Images

 
Western Blot: SYK Antibody (8C1A3) [NBP1-47548] - Analysis using SYK mouse mAb against truncated SYK-His recombinant protein (1) and PMA induced THP-1 cell lysate (2).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clone
8C1A3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

SYK Antibody (8C1A3) Summary

Immunogen
Purified recombinant fragment of SYK(aa296-484) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SYK
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1: 500 - 1:2000
  • ELISA 1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for SYK Antibody (8C1A3)

  • DKFZp313N1010
  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ25043
  • spleen tyrosine kinaseFLJ37489
  • SYK
  • tyrosine-protein kinase SYK

Background

Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Substitution of Tyr-525 and Tyr-526 at the autophosphorylation site of Syk in mCD8-Syk substantially reduced the kinase activity and the binding to PLC-gamma1 SH2(C) in vitro (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB500-519
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IP
AF2685
Species: Hu
Applications: WB, Simple Western, IHC
NB110-60490
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-19097
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
NBP1-78295
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-37585
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC
NBP1-91228
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
NBP2-27104
Species: Hu, Mu, Pm
Applications: WB, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP2-37574
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC
NB500-517
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-46404
Species: Hu
Applications: IHC, IHC-P

Publications for SYK Antibody (NBP1-47548) (0)

There are no publications for SYK Antibody (NBP1-47548).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SYK Antibody (NBP1-47548) (0)

There are no reviews for SYK Antibody (NBP1-47548). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SYK Antibody (NBP1-47548) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional SYK Products

Bioinformatics Tool for SYK Antibody (NBP1-47548)

Discover related pathways, diseases and genes to SYK Antibody (NBP1-47548). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SYK Antibody (NBP1-47548)

Discover more about diseases related to SYK Antibody (NBP1-47548).
 

Pathways for SYK Antibody (NBP1-47548)

View related products by pathway.

PTMs for SYK Antibody (NBP1-47548)

Learn more about PTMs related to SYK Antibody (NBP1-47548).
 

Research Areas for SYK Antibody (NBP1-47548)

Find related products by research area.

Blogs on SYK

There are no specific blogs for SYK, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SYK Antibody (8C1A3) and receive a gift card or discount.

Bioinformatics

Gene Symbol SYK
Entrez