SYK Antibody (8C1A3)

Western Blot: SYK Antibody (8C1A3) [NBP1-47548] - Analysis using SYK mouse mAb against truncated SYK-His recombinant protein (1) and PMA induced THP-1 cell lysate (2).

Product Discontinued

SYK Antibody (8C1A3) Summary

• Immunogen: Purified recombinant fragment of SYK(aa296-484) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SYK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1: 500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for SYK Antibody (8C1A3)

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Substitution of Tyr-525 and Tyr-526 at the autophosphorylation site of Syk in mCD8-Syk substantially reduced the kinase activity and the binding to PLC-gamma1 SH2(C) in vitro (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYK
• Entrez:
  • 2534(Human)