SYK Antibody (720402) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, KO
• Clone: 720402
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

SYK Antibody (720402) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human SYK (SH2D2 Domain)
  Trp168-Cys259
  Accession # P43405
• Specificity: Detects human SYK (SH2D2 Domain) in direct ELISAs. In direct ELISAs, 100% cross-reactivity with the SH2D2 domain of recombinant human (rh) ZAP70 is observed and no cross-reactivity with the SH2 domain of rhCSK is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SYK Antibody (720402) [Alexa Fluor® 750]

• DKFZp313N1010
• EC 2.7.10
• EC 2.7.10.2
• FLJ25043
• spleen tyrosine kinaseFLJ37489
• SYK
• tyrosine-protein kinase SYK

Background

SYK (spleen tyrosine kinase) is a 70-80 kDa cytoplasmic non-receptor protein tyrosine kinase of the protein kinase superfamily and the SYK/ZAP-70 subfamily of proteins. The 635 amino acid (aa) human SYK contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform lacking aa 371-631. Within the second SH2 domain (SH2D2), human SYK shares 94% aa sequence identity with mouse and rat SYK. SYK is widely expressed in hematopoietic cells (notably B lymphocytes), where it couples immunoglobulin receptors to downstream events, such as proliferation, differentiation, and phagocytosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P43405()