SV2A Antibody - BSA Free

Immunoprecipitation:SV2A AntibodyNBP3-29314] -Detection of human SV2A by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SV2A antibody NBP3-29314 (lot NBP3-29314-1) used for IP at 6 µg per reaction. SV2A was also immunoprecipitated by rabbit anti-SV2A antibody BL21512. For blotting immunoprecipitated SV2A, NBP3-29314 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SV2A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SV2A Antibody - BSA Free (NBP3-29314) is a polyclonal antibody validated for use in IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 1 and 50
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SV2A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate pH 7 -8.
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for SV2A Antibody - BSA Free

• KIAA0736
• KIAA0736SV2
• SV2
• SV2A
• synaptic vesicle glycoprotein 2A

Background

SV2A (Synaptic Vesicle protein 2A) is an integral membrane glycoproteins present in synaptic vesicles. SV2s have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SV2A