SUPT16H Antibody

Western Blot: SUPT16H Antibody [NB100-60942] - (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SUPT16H Antibody Summary

• Description: Novus Biologicals Goat SUPT16H Antibody (NB100-60942) is a polyclonal antibody validated for use in WB and ELISA. Anti-SUPT16H Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DLTNKEGKKPEEK corresponding to internal region according to NP_009123.1.
• Epitope: DLTNKEGKKPEEK
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SUPT16H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: WB: Approx. 150 kDa band observed in HeLa lysates (calculated MW of 120 kDa band according to NP_009123.1). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SUPT16H Antibody

• CDC68
• Chromatin-specific transcription elongation factor 140 kDa subunit
• facilitates chromatin remodeling 140 kDa subunit
• Facilitates chromatin transcription complex subunit SPT16
• FACT 140 kDa subunit
• FACT complex subunit SPT16
• FACT140
• FACTp140
• FACTP140FACT
• FLJ10857
• FLJ14010
• FLJ34357
• hSPT16
• SPT16/CDC68
• suppressor of Ty (S.cerevisiae) 16 homolog
• suppressor of Ty 16 homolog (S. cerevisiae)

Background

Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit, the latter of which is the protein encoded by this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUPT16H Antibody (NB100-60942)(2)

Publications using NB100-60942

• Lyu Y, Yang Y, Talwar V et al Hypoxia-inducible factor 1 recruits FACT and RNF20/40 to mediate histone ubiquitination and transcriptional activation of target genes Cell Rep 2024-03-22 [PMID: 38517892] (WB, Human)
• Belotserkovskaya R, Oh S, Bondarenko VA et al. FACT facilitates transcription-dependent nucleosome alteration. Science 2003-08-22 [PMID: 12934006]

Bioinformatics

• Gene Symbol: SUPT16H
• Entrez:
  • 11198(Human)
• Uniprot:
  • NP_009123.1(Human)