Suppressor of Fused Antibody (1B2)

Sandwich ELISA: Suppressor of Fused Antibody (1B2) [H00051684-M01] - Detection limit for recombinant GST tagged SUFU is approximately 0.1ng/ml as a capture antibody.

Proximity Ligation Assay: Suppressor of Fused Antibody (1B2) [H00051684-M01] - Analysis of protein-protein interactions between STK36 and SUFU. HeLa cells were stained with anti-STK36 rabbit purified polyclonal 1:1200 and anti-SUFU mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Product Discontinued

Suppressor of Fused Antibody (1B2) Summary

• Immunogen: SUFU (AAH13291, 181 a.a. ~ 280 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDPQMQPVQTPFGVVTFLQIVGVCTEELHSAQQWNGQGILELLRTVPIAGGPWLITDMRRGETIFEIDPHLQERVDKGIETDGSNLSGVSAKCAWDDLSR
• Localization: Nuclear and cytoplasmic
• Specificity: SUFU - suppressor of fused homolog (Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUFU
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Proximity Ligation Assay
  • Sandwich ELISA
• Application Notes: Antibody reactive against recombinant protein on ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Suppressor of Fused Antibody (1B2)

• SUFUHSUFUXLPRO1280
• suppressor of fused homolog (Drosophila)
• suppressor of fused homolog

Background

SUFU encodes a component of the sonic hedgehog (SHH; MIM 600725)/patched (PTCH; MIM 601309) signaling pathway. Mutations in genes encoding components of this pathway are deleterious for normal development and are associated with cancer-predisposing syndromes (e.g., HPE3, MIM 142945; BCNS, MIM 109400; and GCPS, MIM 175700).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Suppressor of Fused Antibody (H00051684-M01)(1)

Publication using H00051684-M01

• Lee, DY et al. MicroRNA-378 promotes cell survival, tumor growth, angiogenesis by targeting SuFu Fus-1 expression. PNAS 104(51): 20350-20355. 2007-01-01 [PMID: 18077375]

Bioinformatics

• Gene Symbol: SUFU
• Entrez:
  • 51684(Human)
• OMIM:
  • 155(Human)
• Uniprot:
  • AAH13291(Human)