SUMO4 Antibody (JJ085-01)

Western Blot: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Analysis of SUMO4 on different lysates using anti-SUMO4 antibody at 1/1,000 dilution. Positive control: Lane 1: 293T Lane 2: Jurkat

Immunocytochemistry/Immunofluorescence: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Staining SUMO4 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Flow Cytometry: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Analysis of 293 cells with SUMO4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody

Immunocytochemistry/Immunofluorescence: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Staining SUMO4 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Staining SUMO4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: SUMO4 Antibody (JJ085-01) [NBP2-67906] - Staining SUMO4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

SUMO4 Antibody (JJ085-01) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human SUMO4 aa1-105 / 95. (SwissProt: Q6EEV6 Human; SwissProt: P61957 Mouse; SwissProt: P61959 Rat)
• Localization: Nucleus, SUMO ligase complex.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SUMO4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SUMO4 Antibody (JJ085-01)

• dJ281H8.4
• IDDM5
• small ubiquitin-like modifier 4 protein
• Small ubiquitin-like protein 4
• small ubiquitin-related modifier 4
• SMT3 suppressor of mif two 3 homolog 2
• SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 4 (yeast)
• SMT3H4
• SUMO4
• SUMO-4

Background

SUMO4 is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMO4