Recombinant Human SUMO2 Protein

Product Discontinued

Recombinant Human SUMO2 Protein Summary

• Description: Recombinant bioactive protein for Human SUMO2
  
  

  Source:E. coli
  Amino Acid Sequence:(P61956)

• Details of Functionality: Typical in vitro concentrations for conjugate formation is 10-50 uM depending on conditions.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SUMO2
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 11.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM HEPES (pH 8.0), 150 mM NaCl, 1 mM DTT.
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human SUMO2 Protein

• HSMT3
• MGC117191
• sentrin 2
• Sentrin-2
• small ubiquitin-like modifier 2
• small ubiquitin-related modifier 2
• SMT3 (suppressor of mif two 3, yeast) homolog 2
• SMT3 homolog 2
• SMT3 suppressor of mif two 3 homolog 2 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 2 (yeast)
• SMT3A
• SMT3B
• SMT3H2
• SUMO2
• SUMO-2
• SUMO3
• SUMO-3
• Ubiquitin-like protein SMT3A
• ubiquitin-like protein SMT3B

Background

SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation and transcriptional regulation. SUMO1 functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. Still, unlike ubiquitin which targets proteins for degradation, SUMO-2 is involved in a variety of Cellular processes, for example nuclear transport, transcriptional regulation, apoptosis, and protein stability. The active recombinant SUMO-2 is derived from the precursor pro-SUMO-2 (Accession # NM_006937). Human SUMO-2 shares 44% and 86% identity with SUMO-1 and SUMO-3 respectively. SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from UbcH9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1, RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (I?Ba, GLUT1, GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis and protein stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SUMO2