SUMO1 Antibody (SUMO1/1188) [Alexa Fluor™ Plus 594]

• Reactivity: Hu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, ICC/IF
• Clone: SUMO1/1188
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

SUMO1 Antibody (SUMO1/1188) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Recombinant human SUMO1 protein (Uniprot: P63165)
• Localization: Predominantly nuclear with some cytoplasmic
• Specificity: This monoclonal antibody is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SUMO1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Predicted to show a broad species reactivity.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SUMO1 Antibody (SUMO1/1188) [Alexa Fluor™ Plus 594]

• DAP1
• GAP modifying protein 1
• GAP-modifying protein 1
• GMP1SMT3CSMT3H3OFC10UBL1PIC1
• PIC1
• SENP2
• Sentrin
• small ubiquitin-related modifier 1
• SMT3 homolog 3
• SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 1 (yeast)
• SMT3
• SMT3C
• SMT3H3
• SUMO1
• SUMO-1
• Ubiquitin-homology domain protein PIC1
• ubiquitin-like 1 (sentrin)
• Ubiquitin-like protein SMT3C
• Ubiquitin-like protein UBL1
• UBL1

Background

Sumo 1 is a ubiquitin-like protein which binds to a wide range of target proteins. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved in targeting RANGAP1 to the nuclear pore complex protein RANBP2. SUMO 1 is also known to form a complex with RanGAP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SUMO1