SUMO1 Antibody (SPM571) [HRP]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM571
Clonality
Monoclonal
Host
Mouse

SUMO1 Antibody (SPM571) [HRP] Summary

Immunogen
Recombinant human SUMO1 protein
Localization
Predominantly nuclear with some cytoplasmic
Specificity
This MAb is specific to SUMO-1 and shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2 and SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SUMO1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
HRP
Format
HRP
Buffer
PBS
Preservative
No Preservative
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Readout System

Reactivity Notes

Shows broad species reactivity.

Alternate Names for SUMO1 Antibody (SPM571) [HRP]

  • DAP1
  • GAP modifying protein 1
  • GAP-modifying protein 1
  • GMP1SMT3CSMT3H3OFC10UBL1PIC1
  • PIC1
  • SENP2
  • Sentrin
  • small ubiquitin-related modifier 1
  • SMT3 homolog 3
  • SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 1 (yeast)
  • SMT3
  • SMT3C
  • SMT3H3
  • SUMO1
  • SUMO-1
  • Ubiquitin-homology domain protein PIC1
  • ubiquitin-like 1 (sentrin)
  • Ubiquitin-like protein SMT3C
  • Ubiquitin-like protein UBL1
  • UBL1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SUMO1 Antibody (NBP2-34805H) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on SUMO1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol SUMO1
Entrez
Uniprot

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