![Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NB100-1312] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize/SUMO-Activating-Enzyme-E1-SAE1-Antibody-Western-Blot-NB100-1312-img0003.jpg "Western Blot: SUMO Activating Enzyme E1 (SAE1) Antibody [NB100-1312] - (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.")
| Reactivity | Hu, Bv, CaSpecies Glossary |
| Applications | WB, ELISA |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence MKGNGIVECLGPK corresponding to C-Terminus according to NP_005491.1. |
| Predicted Species | Bovine (100%), Canine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | SAE1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | WB: Approx. 40 kDa band observed in Jurkat and 293 lysates (predicted MW of 40 kDa band according to NP_005491). |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-1312 | Applications | Species |
|---|---|---|
| Desterro JM, Rodriguez MS, Kemp GD, Hay RT. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J Biol Chem 1999-04-09 [PMID: 10187858] |
Secondary Antibodies |
Isotype Controls |
Research Areas for SUMO Activating Enzyme E1 (SAE1) Antibody (NB100-1312)Find related products by research area.
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| Gene Symbol | SAE1 |
| Entrez |
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| Uniprot |
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