SUCLA2 Overexpression Lysate (Native)

Images

 
Western Blot: SUCLA2 Overexpression Lysate (Adult Normal) [NBL1-16594] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SUCLA2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SUCLA2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC205212 and based on accession number NM_003850. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens succinate-CoA ligase, ADP-forming, beta subunit (SUCLA2), nuclear gene encoding mitochondrial protein, mRNA.
Gene
SUCLA2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
44.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SUCLA2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SUCLA2 Overexpression Lysate (Native)

  • A-BETA
  • ATP-specific succinyl-CoA synthetase subunit beta
  • beta subunit
  • EC 6.2.1
  • EC 6.2.1.5
  • mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta
  • MTDPS5
  • Renal carcinoma antigen NY-REN-39
  • succinate-CoA ligase, ADP-forming, beta subunit
  • succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial

Background

Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SUCLA2 Lysate (NBL1-16594) (0)

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Video Protocols

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Bioinformatics

Gene Symbol SUCLA2