SUB1 Antibody

Western Blot: SUB1 Antibody [NB100-59775] - Analysis using rat cardiomyocyte lysate with anti-SUB1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: SUB1 Antibody [NB100-59775] - Sample: FFPE section of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- PC4 used at a dilution of 1:200 (1ug/ml) and 1:1,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: SUB1 Antibody [NB100-59775] - Detection of human SUB1/PC4 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SUB1 antibody NB100-59775 used for WB at 0.04 ug/ml (A) and 0.1 ug/ml (B). SUB1 was immunoprecipitated by rabbit anti-SUB1 antibody NB100-59774, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).

• Reactivity: Hu, Mu, Rt, Ch, Pm
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SUB1 Antibody Summary

• Description: Novus Biologicals Rabbit SUB1 Antibody (NB100-59775) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-SUB1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 77 and 127 of human positive cofactor 4 using the numbering given in entry NP_006704.2 (GeneID 10923).
• Predicted Species: Chicken (100%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SUB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Control:

  SUB1 Overexpression Lysate

Reactivity Notes

Orangutan (100%). Rat reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SUB1 Antibody

• activated RNA polymerase II transcription cofactor 4
• activated RNA polymerase II transcriptional coactivator p15
• MGC102747
• p15
• PC4p14P15
• Positive cofactor 4
• RPO2TC1
• SUB1 homolog (S. cerevisiae)
• SUB1 homolog

Background

PC4 is a multifunctional transcriptional coactivator that plays an important role in transcription, repair, and replication. PC4 interacts with general transcription factors, DNA repair factors, and mRNA processing factors to influence transcription initiation, coactivation, polyadenylation, transcription termination, and transcriptional repression. PC4 has been demonstrated to be a DNA binding protein and a bona fide component of chromatin with chromatin-compacting ability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SUB1 Antibody (NB100-59775)(2)

Publications using NB100-59775

• Huang R, Hu Z, Chen X Et al. The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021-08-10 [PMID: 34378353] (Western Blot, Human)
• Lin SJ, Chang KP, Hsu CW et al. Low-molecular-mass secretome profiling identifies C-C motif chemokine 5 as a potential plasma biomarker and therapeutic target for nasopharyngeal carcinoma. J Proteomics 2013-09-27 [PMID: 24080422] (WB, Human)

Review for SUB1 Antibody (NB100-59775) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Shinichi Oka WB Rat 09/08/2022

Summary

• Application: Western Blot
• Sample Tested: primary neonatal ventricular cardiomyocytes
• Species: Rat
• Lot: A1

Comments

• Comments: ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: SUB1
• Entrez:
  • 10923(Human)
• Uniprot:
  • NP_006704.2(Human)