STK39 Antibody

Images

 
Western Blot: STK39 Antibody [NBP1-51955] - Analysis in Mouse (A) and Rat (B) Brain (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: STK39 Antibody [NBP1-51955] - (3.8ug/ml) Staining of Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: STK39 Antibody [NBP1-51955] - (0.5ug/ml) Staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

STK39 Antibody Summary

Immunogen
Peptide with sequence C-SQEKSRRVKEENPE corresponding to internal region according to NP_037365.2.
Epitope
C-SQEKSRRVKEENPE
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STK39
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin 3 - 5 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 65 kDa band observed in human brain (cerebellum) lysates and in Jurkat lysates (calculated MW of 59.5 kDa band according to NP_037365.2). Approx. 75 kDa band observed in mouse and rat brain lysates (calculated MW of 60.3 kDa band according to mouse NP_058562.1). IHC-P: Human cerebral cortex shows textured staining in the neurons.
Publications
Read Publication using NBP1-51955.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STK39 Antibody

  • DCHTsmall intestine SPAK-like kinase
  • DKFZp686K05124
  • EC 2.7.11
  • EC 2.7.11.1
  • PASK
  • proline-alanine-rich STE20-related kinase
  • serine threonine kinase 39
  • Serine/threonine-protein kinase 39
  • SPAKserine threonine kinase 39 (STE20/SPS1 homolog, yeast)
  • STE20/SPS1 homolog
  • STE20/SPS1-related proline-alanine-rich protein kinase
  • Ste20-like protein kinase
  • Ste-20-related kinase

Background

This gene encodes a serine/threonine kinase that is thought to function in the cellular stress response pathway. The kinase is activated in response to hypotonic stress, leading to phosphorylation of several cation-chloride-coupled cotransporters. The catalytically active kinase specifically activates the p38 MAP kinase pathway, and its interaction with p38 decreases upon cellular stress, suggesting that this kinase may serve as an intermediate in the response to cellular stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for STK39 Antibody (NBP1-51955)(1)

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Video Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol STK39
Entrez
Uniprot