STK35 Antibody - BSA Free

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Western Blot: STK35 Antibody [NBP2-88372] - WB Suggested Anti-STK35 Antibody. Titration: 1.0 ug/ml. Positive Control: HepG2 Whole Cell

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
0.5 mg/ml

Order Details

STK35 Antibody - BSA Free Summary

Description
Novus Biologicals Rabbit STK35 Antibody - BSA Free (NBP2-88372) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
Immunogen
The immunogen is a synthetic peptide directed towards the N-terminal region of Human STK35. Peptide sequence: PPRPRAGRRDEAGGARAAPLLLPPPPAAMETGKDGARRGTQSPERKRRSP The peptide sequence for this immunogen was taken from within the described region.
Clonality
Polyclonal
Host
Rabbit
Gene
STK35
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Affinity purified

Alternate Names for STK35 Antibody - BSA Free

  • bA550O8.2
  • CLIK-1
  • CLIK1Serine/threonine-protein kinase 35 L1
  • CLP-36 interacting kinase
  • CLP-36-interacting kinase 1
  • EC 2.7.11
  • EC 2.7.11.1
  • PDIK1
  • PDLIM1-interacting kinase 1
  • serine threonine kinase 35 long form
  • serine/threonine kinase 35
  • serine/threonine-protein kinase 35
  • STK35L1

Background

STK35 associates with the PDZ-LIM protein CLP36 through the LIM domain and undergoes autophosphorylation in vitro. STK35 localizes predominantly to the nucleus; however, cotransfection of STK35 with CLP36 demonstrated redistribution of STK35 to the cytoplasm and into actin stress fibers, where it colocalizes with CLP36. The presence of STK35 disrupted the periodic staining pattern of CLP36 in these fibers. It is suggested that CLP36 specifically targets STK35 to actin stress fibers and that STK35 may represent a regulator of the actomyosin cytoskeleton in nonmuscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK35