STK25 Antibody

Images

 
Western Blot: STK25 Antibody [NBP1-32670] - Sample (30 ug of whole cell lysate)A: Hela S310% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: STK25 Antibody [NBP1-32670] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: STK25 Antibody [NBP1-32670] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:100 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.38 mg/ml

Order Details

STK25 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human STK25. The exact sequence is proprietary.
Localization
Cytoplasm, Golgi apparatus
Predicted Species
Mouse (99%), Rat (99%), Xenopus (91%), Bovine (99%), Canine (98%), Chicken (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
STK25
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (91%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.38 mg/ml
Purity
Immunogen affinity purified

Alternate Names for STK25 Antibody

  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine kinase 25 (Ste20, yeast homolog)
  • serine/threonine kinase 25
  • serine/threonine-protein kinase 25
  • SOK-1
  • SOK1DKFZp686J1430
  • Ste20, yeast homolog
  • Ste20/oxidant stress response kinase 1
  • sterile 20 (oxidant stress response kinase 1; yeast Sps1/Ste20-related kinase1)
  • Sterile 20/oxidant stress-response kinase 1
  • YSK1yeast)

Background

Mitogen activated protein kinase cascades have been conserved throughout evolution. In mammals, these cascades allow responses to complex stimuli such as growth factors and inflammatory cytokines. In yeast, STK25 functions upstream of the MAPK cascade.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol STK25