STAT2 [p Tyr689] Antibody [Fluorescein]

Daudi human Burkitt's lymphoma cell line untreated (light orange filled histogram) or treated with Recombinant Human IFN-aA (11100-1) (dark orange filled histogram) was stained with Rabbit Anti-Human Phospho-STAT2 (Y689) Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2890F) or isotype control antibody (IC105F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Product Discontinued

STAT2 [p Tyr689] Antibody [Fluorescein] Summary

• Immunogen: Phosphopeptide containing the human STAT2 Y689 site
• Modification: p Tyr689
• Specificity: Detects human STAT2 when phosphorylated at Y689 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: STAT2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Intracellular Staining by Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for STAT2 [p Tyr689] Antibody [Fluorescein]

• interferon alpha induced transcriptional activator
• ISGF-3
• MGC59816
• P113
• signal transducer and activator of transcription 2
• signal transducer and activator of transcription 2, 113kD
• signal transducer and activator of transcription 2, 113kDa
• STAT113
• STAT2

Background

STAT2 (Signal Transducer and Activator of Transcription #2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with Type I ( alpha - and beta -) Interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for STAT2 Antibody (IC2890F)(1)

Publication using IC2890F

• Thacker SG, Berthier CC, Mattinzoli D, Rastaldi MP, Kretzler M, Kaplan MJ The detrimental effects of IFN-alpha on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction. J. Immunol., 2010-08-30;185(7):4457-69. 2010-08-30 [PMID: 20805419] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: STAT2