Mouse STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line

Product Discontinued

Mouse STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line Summary

• Description: The STAT1 / LUCPorter reporter cell line can be used for screening of agonists, antagonists or signaling inhibitors related with the STAT1-involved signaling pathways. The cell line is designed to measure the transcriptional activity of STAT1.
  
  As a transcription factor, Signal Transducer and Activator of Transcription-1 (STAT1) is activated through phosphorylation at tyrosine 701 in response to various cytokines and growth factors such as IFN-alpha, IFN-gamma, IL-6, EGF and PDGF. The phosphorylated STAT1 forms homodimers or heterodimers with STAT3, and the dimers translocate to nucleus in which DNA binding/promoter induction occurs. Unlike STAT3, STAT1 has been known to function as a tumor suppressor, triggering anti-proliferative and pro-apoptotic responses while enhancing anti-tumor immunity. On the other hand, a recent study has shown that STAT1 activity is elevated in human and mouse breast cancers, promoting breast cancer progression. The STAT1 induction by IFN-gamma is shown in Figure 1. In the test, IFN-gamma induced the transcriptional activity of STAT1 in a dose response manner (Figure 1).
  
  Contents : 3 ~ 4 x 10^6 cells
  Biosafety Level : 2
• Immunogen: The STAT1 / LUCPorter™ reporter cell line is a stably transfected RAW 264.7 cell line which expresses an optimized Renilla luciferase reporter gene (RenSP) under the transcriptional control of the interferon (IFN)-gamma activation sequence-based STAT1 response element.
• Target Species: Mouse
• Selection Agent: Puromycin
• Growth Properties: Adherent Morphology: Macrophage
• RCL Type: Luciferase - (LUCPorter™)
• Host: RAW264.7
• Gene: STAT1

Applications/Dilutions

• Dilutions:
  • Ligand Activation
• Readout System:
  • LightSwitch Luciferase Assay Reagent NBP2-32066

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 3 ug/ml puromycin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
No eating, drinking or smoking while handling the STAT1 reporter line.
Wash hands after handling the STAT1 reporter cell line and before leaving the lab.
Decontaminate work surface with disinfectant or 70% ethanol before and after working with the reporter line.
All waste should be considered hazardous.
Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Mouse STAT1 Luciferase - (LUCPorter™) Stable Reporter Cell Line

• CANDF7
• DKFZp686B04100
• ISGF-3
• ISGF-3,91kD
• signal transducer and activator of transcription 1, 91kDa
• signal transducer and activator of transcription-1
• STAT1
• STAT91

Background

STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-a, IFN-b, IFN-g and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1). STAT1 has two forms, the 91 kDa STAT1a and the 84 kDa STAT1b which are encoded by the same gene with splicing variant.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAT1