| Immunogen | FM9 melanoma cells. |
| Specificity | Reacts specifically with human GD3 ganglioside (Cheresh et al., 1984). No cross-reactivity with other gangliosides purified from neuroblastoma or melanoma cells. |
| Isotype | IgG3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | ST8SIA1 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
|
| Application Notes | Immunohistochemistry (frozen tissue sections) Immunofluorescence of cells in culture. Flow cytometry: Lysis of GD3 positive (melanoma) cells in presence of complement (Cheresh et al., 1985). Induction of antibody dependent cellular cyotoxicity (ADCC) {Ortaldo JR et al 1987 J Immunol 138(10):3566-3572. TLC analysis: Sjoberg, ER (1995) J. Biol. Chem. 270(7):2921-2930.{http://www.jbc.org/cgi/content/full/270/7/2921}. IHC-P reported in a verified customer review. |
|
| Reviewed Applications |
|
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 20 mM Phosphate (pH 7.6), 0.25 M NaCl |
| Preservative | 0.1% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A or G purified |
| Images | Ratings | Applications | Species | Date | Details | ||||
|---|---|---|---|---|---|---|---|---|---|
Enlarge |
reviewed by:
Verified Customer |
IF | 05/21/2014 |
Summary
|
Secondary Antibodies |
Isotype Controls |
Research Areas for ST8 alpha-2,8-Sialyltransferase 8A/ST8SIA1/Ganglioside GD3 Antibody (NBP2-29826)Find related products by research area.
|
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | ST8SIA1 |