ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of ST6GAL1 expression in transfected 293T cell line by ST6GAL1 polyclonal antibody. A: Non-transfected lysate. B: ST6GAL1 transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Human hepatocellular carcinoma. CD75 antibody [N2C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemistry-Paraffin: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Analysis of paraffin-embedded OVCA xenograft, using antibody at 1:500 dilution.

Western Blot: ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody [NBP2-15817] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CD75 antibody [N2C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

• Reactivity: Hu, Pm, RM
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free (NBP2-15817) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CD75. The exact sequence is proprietary.
• Localization: Golgi apparatus; Golgi stack membrane; Single-pass type II membrane protein; Secreted
• Predicted Species: Chimpanzee (99%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST6GAL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Bovine (88%), Mouse (87%), Rat (87%), Sheep (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody - BSA Free

• Alpha 2,6-ST
• B-cell antigen CD75
• beta-galactoside alpha-2,6-sialyltransferase 1
• CD75
• CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase
• CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1
• EC 2.4.99.1
• MGC48859
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
• sialyltransferase 1 (beta-galactoside alpha-2,6-sialytransferase)
• Sialyltransferase 1
• SIAT1
• SIAT1Alpha 2,6-ST 1
• ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
• ST6Gal I
• ST6GAL1
• ST6GalI
• ST6N

Background

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ST6GAL1
• Uniprot:
  • P15907()