SSH2 Antibody - Azide and BSA Free

Western Blot: SSH2 Antibody [H00085464-D01P] - Analysis of SSH2 expression in transfected 293T cell line by SSH2 polyclonal antibody.Lane 1: SSH2 transfected lysate(19.90 KDa).Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SSH2 Antibody - Azide and BSA Free Summary

• Immunogen: SSH2 (AAH11636.2, 1 a.a. - 178 a.a.) full-length human protein. MIPYFSANAVISQNAINQLISESFLTVKGAALFLPRGNGSSTPRISHRRNKHAGDLQQHLQAMFILLRPEDNIRLAVRLESTYQNRTRYMVVVSTNGRQDTEESIVLGMDFSSNDSSTCTMGLVLPLWSDTLIHLDGDGGFSVSTDNRVHIFKPVSVQAMWVDRDSRNKHCDVLLVEE
• Specificity: SSH2 - slingshot homolog 2 (Drosophila),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSH2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25568344)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSH2 Antibody - Azide and BSA Free

• EC 3.1.3.16
• EC 3.1.3.48
• hSSH-2L
• KIAA1725protein phosphatase Slingshot homolog 2
• MGC78588
• slingshot 2
• slingshot homolog 2 (Drosophila)
• SSH-2
• SSH2L
• SSH-2L
• SSH-like protein 2

Background

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSH2 Antibody (H00085464-D01P)(1)

Publication using H00085464-D01P

• Xu X, Gera N, Li H et al. GPCR-Mediated PLCbeta-gamma/PKCbeta/PKD Signaling Pathway Regulates the Cofilin Phosphatase Slingshot 2 in Neutrophil Chemotaxis Mol. Biol. Cell. 2015-01-07 [PMID: 25568344] (WB, Mouse)
  
  Details:
  SSH2 antibody used for WB analysis of anti-GFPagarose - immunoprecipitates derived from RAW264.7 cells transiently expressing GFP only or GFP-tagged PKD1 protein (FIGURE 7A).

Bioinformatics

• Gene Symbol: SSH2
• Entrez:
  • 85464(Human)
• Uniprot:
  • AAH11636.2(Human)