SSH3 Antibody (6F9) - Azide and BSA Free

Images

 
Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - SSH3 monoclonal antibody (M01), clone 6F9 Analysis of SSH3 expression in A-431.
Immunocytochemistry/ Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ~ 10 ug/ml.
Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody (M01), clone 6F9.Lane 1: SSH3 transfected lysate(73 KDa).Lane 2: Non-transfected ...read more
Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SSH3 monoclonal antibody.
Sandwich ELISA: SSH3 Antibody (6F9) [H00054961-M01] - Detection limit for recombinant GST tagged SSH3 is approximately 0.3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IP
Clone
6F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free

Order Details

SSH3 Antibody (6F9) - Azide and BSA Free Summary

Immunogen
SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPH
Specificity
SSH3 - slingshot homolog 3 (Drosophila)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SSH3
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.
Publications
Read Publication using
H00054961-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSH3 Antibody (6F9) - Azide and BSA Free

  • EC 3.1.3.16
  • EC 3.1.3.48
  • FLJ10928
  • FLJ20515
  • FLJ42240
  • hSSH-3L
  • protein phosphatase Slingshot homolog 3
  • slingshot 3
  • slingshot homolog 3 (Drosophila)
  • SSH3
  • SSH3L
  • SSH-3L
  • SSH-like protein 3

Background

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SSH3 Antibody (H00054961-M01)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ELISA.


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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol SSH3
Entrez
OMIM
Uniprot