SSH3 Antibody (6F9)

Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - SSH3 monoclonal antibody (M01), clone 6F9 Analysis of SSH3 expression in A-431.

Immunocytochemistry/Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on A-431 cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ~ 10 ug/ml.

Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody (M01), clone 6F9.Lane 1: SSH3 transfected lysate(73 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SSH3 monoclonal antibody.

Sandwich ELISA: SSH3 Antibody (6F9) [H00054961-M01] - Detection limit for recombinant GST tagged SSH3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, S-ELISA
• Clone: 6F9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SSH3 Antibody (6F9) Summary

• Immunogen: SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPH
• Specificity: SSH3 - slingshot homolog 3 (Drosophila)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SSH3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for SSH3 Antibody (6F9)

• EC 3.1.3.16
• EC 3.1.3.48
• FLJ10928
• FLJ20515
• FLJ42240
• hSSH-3L
• protein phosphatase Slingshot homolog 3
• slingshot 3
• slingshot homolog 3 (Drosophila)
• SSH3
• SSH3L
• SSH-3L
• SSH-like protein 3

Background

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSH3 Antibody (H00054961-M01)(1)

Publication using H00054961-M01

• Kassim SH, Jordan J, Schreiter J et al. Systematic identification of novel SLE related autoantibodies responsible for type I IFN production in human plasmacytoid dendritic cells. Cell Immunol. 2013-08-06 [PMID: 23973875] (ELISA, Human)

Bioinformatics

• Gene Symbol: SSH3
• Entrez:
  • 54961(Human)
• OMIM:
  • 606780(Human)
• Uniprot:
  • NP_060327(Human)