SSH1 Antibody

Western Blot: SSH1 Antibody [NBP3-23410] - Western blot of human recombinant SSH1 untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1 (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1 (Ser-978) (lanes 3, 4, 7, & 8).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SSH1 Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1. This sequence has 100% homology with similar regions of mouse and bovine SSH1, and has low homology to SSH2L and SSH3L. [UniProt# Q8WYL5]
• Specificity: The antibody detects a 150 kDa protein corresponding to the molecular mass of SSH1 on SDS-PAGE immunoblots of PC12 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:250
  • Immunohistochemistry 1:100
  • Western Blot 1:1000
• Theoretical MW: 150 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for SSH1 Antibody

• EC 3.1.3.16
• EC 3.1.3.48
• FLJ21928
• hSSH-1L
• KIAA1298FLJ38102
• protein phosphatase Slingshot homolog 1
• slingshot 1
• slingshot homolog 1 (Drosophila)
• SSH1L
• SSH-1L
• SSH-like protein 1

Background

SSH1, also known as slingshot, is a phosphatase that functions to activate the actin severing protein, ADF/cofilin, and influence actin dynamics. There are three members of the slingshot family, SSH1, SSH2, and SSH3. SSH1 has been shown to function downstream of alpha6beta4 integrin/Rac1 signaling in conjunction with 14-3-3 association.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SSH1