SSEA-1 Antibody (MC-480) [NL557]

The stem cell marker SSEA-1 was visualized in live D3 mouse embryonic stem cells, grown on irradiated mouse embryonic fibroblasts (Catalog # PSC001), using a GloLIVE NL557-conjugated Anti-Human/Mouse SSEA-1 Monoclonal Antibody (Catalog # NLLC2155R; red). The nuclei were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-1 in mouse stem cells is an indicator of pluripotency.

• Reactivity: Hu, Mu
• Applications: ICC/IF
• Clone: MC-480
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: NL557

SSEA-1 Antibody (MC-480) [NL557] Summary

• Additional Information: 50X Concentration, Azide Free
• Specificity: Detects human and mouse SSEA-1.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SSEA-1 Antibody (MC-480) [NL557]

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• FucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

Stage-Specific Embryonic Antigen-1 (SSEA-1), an antigenic epitope defined as a Lewis x carbohydrate structure, is expressed during early mouse embryogenesis on murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES), and murine and human germ cells. Expression of SSEA-1 is down-regulated following differentiation of murine EC and ES cells. In contrast, the differentiation of human EC and ES cells is accompanied by an increase in SSEA-1 expression (1, 2).

1. Solter, D. and Knowles, B.B. (1978) Proc. Natl. Acad. Sci. USA 75:5565.
2. Fox, N. et al. (1983) Cancer Res. 43:669.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SSEA-1 Antibody (NLLC2155R)(1)

Publication using NLLC2155R

• Jin, G;Porello, EAL;Zhang, J;Lim, B; Heterogeneous Sox2 transcriptional dynamics mediate pluripotency maintenance in mESCs in response to LIF signaling perturbations bioRxiv : the preprint server for biology 2025-03-17 [PMID: 40166162] (Cell Culture, Mouse)

Bioinformatics

• Gene Symbol: FUT4