SSEA-1 Antibody (FUT4/1478R)

Immunohistochemistry-Paraffin: Neutrophils Antibody (FUT4/1478R) [NBP2-53367] - Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Recombinant Rabbit Monoclonal Antibody (FUT4/1478R).

Product Discontinued

SSEA-1 Antibody (FUT4/1478R) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Purified human neutrophils
• Localization: Cell surface and granular paranuclear
• Marker: Reed-Sternberg Cell Marker
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: FUT4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2 - 4ug/million cells
  • Immunocytochemistry/ Immunofluorescence 2 - 4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
• Application Notes: IHC-P:Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes
• Theoretical MW: 220 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for SSEA-1 Antibody (FUT4/1478R)

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD15/Lewis X Antibody (NBP2-53367)(2)

Publications using NBP2-53367

• Castillo EJ, Messer JG, Abraham AM, et al. Preventing or Controlling Periodontitis Reduces the Occurrence of Osteonecrosis of the Jaw (ONJ) in Rice Rats (Oryzomys palustris) Bone 2021-01-27 [PMID: 33515777]
• Curtiellas-Pinol V, Ventura-Juarez J, Ruiz-Baca E, Romo-Lozano Y. Morphological changes and phagocytic activity during the interaction of human neutrophils with Sporothrix schenckii: An in vitro model Microb. Pathog. 2019-01-30 [PMID: 30710673] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: FUT4