SSEA-1 Antibody (C3D-1)

Product Discontinued

SSEA-1 Antibody (C3D-1) Summary

• Immunogen: Purified neutrophils from normal human peripheral blood.
• Localization: Cell membrane
• Specificity: The specificity of this antibody is similar to antibodies Leu-M1, TU 9 and VIM-D5, recognizing an epitope involving the carbohydrate sequence 3-fucosyl-N-acetyllacetosamine. This antibody reacts with an antigen located on mature granulocytes and on Hodgkin and Reed-Sternberg cells.
• Isotype: IgM Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:25
• Application Notes: IHC-Paraffin requires high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 or Tris-EDTA buffer, pH 9.0 prior to immunostaining.

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tissue culture supernatant
• Preservative: Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for SSEA-1 Antibody (C3D-1)

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FUT4
• Entrez:
  • 2526(Human)
• Uniprot:
  • P22083(Human)