SSEA-1 Antibody (BRA4F1)

Product Discontinued

SSEA-1 Antibody (BRA4F1) Summary

• Immunogen: The antibody reacts with the CD15 antigen.
• Specificity: This CD15 (BRA4F1) specifically detects CD15. CD15 contains the pentasaccharide lacto-N-fucopentatose III and is particularly expressed on granulocytes and mature neutrophils and on a wide variety of tumor cells including myeloid leukemia, breast, colorectal, and lung cancer cells. This CD15 (BRA4F1) was clustered at the IVth International Workshop on Leucocyte Differentiation Antigens. Source: A BALB/c mouse was immunized with K562 (an erythroid-myeloid leukemia cell line). Splenocytes were fused with mouse myeloma SP2.0-Ag14 cells.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: FUT4
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 0.5 - 1.0 ug / million cells in 0.1 mL
  • Immunocytochemistry/ Immunofluorescence 0.5 - 1.0 ug/mL
  • Immunohistochemistry 1.0 - 2.0 ug/mL
  • Immunohistochemistry-Frozen 1.0 - 2.0 ug/mL
  • Immunohistochemistry-Paraffin 1.0 - 2.0 ug/mL
• Application Notes: This CD15 (BRA4F1) antibody has been tested on frozen and paraffin embedded tissue sections and in ELISA and in flow cytometry.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: IgM purified

Alternate Names for SSEA-1 Antibody (BRA4F1)

• alpha-(1,3)-fucosyltransferase
• CD15
• EC 2.4.1
• EC 2.4.1.-
• EC 2.4.1.65
• ELAM-1 ligand fucosyltransferase
• ELFT
• FCT3AELAM ligand fucosyltransferase
• fucosyltransferase 4 (alpha (1,3) fucosyltransferase, myeloid-specific)
• Fucosyltransferase 4
• Fucosyltransferase IV
• FUC-TIV
• fucT-IV
• FUTIV
• Galactoside 3-L-fucosyltransferase
• Lewis X
• LeX
• SSEA1
• SSEA-1
• stage-specific embryonic antigen 1

Background

The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for SSEA-1 Antibody (NBP1-42198). (Showing 1 - 1 of 1 FAQ).

1. Does the CD15 antibody (NBP1-42198) specifically recognize the pentasaccharide lacto-N-fucopentatose III?
   • We have not evaluated any potential cross-reactivity between NBP1-42198 and lacto-N-fucopentatose III specifically.

Bioinformatics

• Gene Symbol: FUT4