SREBP2 Antibody [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ChIP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 405

SREBP2 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human SREBP2
  Leu242-Asp450
  Accession # Q12772
• Specificity: Detects human SREBP2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SREBP1B and rhSREBP1C is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SREBP2 Antibody [Alexa Fluor® 405]

• bHLhd2
• bHLHd2sterol regulatory element-binding protein 2
• Class D basic helix-loop-helix protein 2
• SREBF2
• SREBP2
• SREBP2SREBP-2
• sterol regulatory element binding transcription factor 2
• Sterol regulatory element-binding transcription factor 2

Background

SREBP2 (Sterol Regulatory Element-Binding Protein 2; also bHLHD2 and SREBF2) is a 120-125 kDa member of the SREBP family of proteins. It is ubiquitously expressed and found in the intracellular membrane fraction of cells. SREBP2 is a transcriptional factor initially embedded in the ER as an inactive precursor associated with SCAP. When necessary, SCAP mediates SREBP2 transfer to the Golgi, where two resident proteases remove the N-terminus from SREBP2, and the N-terminus is transported into the nucleus. Here, SREBP2 acts as a transcription factor, activating the LDLR and cholesterol synthesis genes. The human SREBP2 precursor is an 1141 amino acid (aa) two transmembrane protein whose N- and C-termini are cytoplasmic. The two cytoplasmic domains span aa 1-479 and 555-1141, respectively. Proteolytic cleavage between Leu484-Cys485 generates the 64-66 kDa SREBP2 transcription factor. This fragment contains a bHLH DNA binding domain (aa 330-380) and one Leu zipper region (aa 381-401). Homodimerization of SREBP2 is necessary for nuclear translocation. There is one potential isoform that shows a deletion of aa 274-276 coupled to a 96 aa substitution for aa 580-1141. A second isoform (known in rodent) shows a premature truncation after Val463 and runs at 55 kDa in SDS-PAGE. Over aa 242-450, human SREBP2 shares 97% aa identity with mouse SREBP2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.