Src Antibody (5A18)

Images

 
Western Blot: Src Antibody (5A18) [NBP1-19188] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot. From a ...read more
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human prostate tissue stained with Src, mAb (5A18) at 20 ug/ml.
Western Blot: Src Antibody (5A18) [NBP1-19188] - Western blot analysis of Src using 20 ug of whole cell lysate (Lane 2=HeLa, Lane 3=3T3, Lane 4=PC12) probed with with Src, mAb (5A18) at 1 ug/ml.
Immunohistochemistry: Src Antibody (5A18) [NBP1-19188] - IHC Analysis: Human adrenal tissue stained with Src, mAb (5A18) at 20 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, IHC-P
Clone
5A18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Src Antibody (5A18) Summary

Immunogen
Recombinant human full length c-Src
Specificity
Src (5A18)
Clonality
Monoclonal
Host
Mouse
Gene
SRC
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • Simple Western
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
WB validation from a verified customer review.
Simple Western reported by an internal validation. Separated by Charge, antibody dilution of 1:50.
Control
Src Knockout HeLa Cell Lysate
Src Knockout HeLa Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-19188 in the following applications:

Publications
Read Publication using
NBP1-19188 in the following applications:

  • WB
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Src Antibody (5A18)

  • ASV
  • c-Src
  • EC 2.7.10
  • EC 2.7.10.2
  • pp60c-src
  • Rous sarcoma
  • RSVgp4
  • Src
  • tyrosine kinase pp60c-src
  • tyrosine-protein kinase SRC-1
  • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)

Background

Src is a member of the tyrosine kinase family that was originally identified as the oncogene of Rous sarcoma virus. The catalytic domain contains a tyrosine autophosphorylation site that increases kinase activity when phosphorylated. The Src family of tyrosine kinase is involved in a variety of receptor-induced signal transduction pathways such as immune recognition rec

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, IHC, IHC-P

Publications for Src Antibody (NBP1-19188)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Reviews for Src Antibody (NBP1-19188) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP1-19188:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
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Images Ratings Applications Species Date Details
Western Blot Src NBP1-19188
Enlarge
5
reviewed by:
Haiquan Lu
WB Human 10/11/2018
View

Summary

ApplicationWestern Blot
Sample TestedBreast cancer cells
SpeciesHuman
Lot12180625
Western Blot Src NBP1-19188
Enlarge
5
reviewed by:
Hongxia Hu
WB Human 09/12/2012
View

Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Src Antibody (NBP1-19188) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Bioinformatics Tool for Src Antibody (NBP1-19188)

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Visit Tool

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Research Areas for Src Antibody (NBP1-19188)

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Blogs on Src.

Androgen Receptor: What Makes a Man?
Steroid receptors (SRs) are a superfamily of ligand-dependent nuclear transcription factors that activate responsive genes response to hormone. Androgen receptors (ARs) are found in a wide variety of tissues, including reproductive organs, central ner...  Read full blog post.

The Heat is On: Heat Shock Proteins and the Link to Cancer
Novus Biologicals offers an extensive antibody catalog targeting heat shock proteins (HSPs). A large protein group covering a number of families, the HSPs are functionally related by their dramatic upregulation in response to stress. Stress triggers m...  Read full blog post.

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Haiquan Lu
10/11/2018
Application: WB
Species: Human

Hongxia Hu
09/12/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol SRC
Entrez
Uniprot