SPRR3 Antibody - BSA Free

Immunoprecipitation: SPRR3 Antibody - BSA Free [NBP3-45827] - IP Result of anti-SPRR3 (IP:, 3ug; Detection: 1:500) with COLO 320 cells lysate 1720ug.

Immunocytochemistry/ Immunofluorescence: SPRR3 Antibody - BSA Free [NBP3-45827] - Immunofluorescent analysis of HeLa cells using (SPRR3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L)

Western Blot: SPRR3 Antibody - BSA Free [NBP3-45827] - HeLa cells were subjected to SDS PAGE followed by western blot with (SPRR3 antibody) at dilution of 1:1000

Immunohistochemistry: SPRR3 Antibody - BSA Free [NBP3-45827] - Immunohistochemistry of paraffin-embedded human colon cancer using (SPRR3 antibody) at dilution of 1:100

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPRR3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPRR3 Antibody - BSA Free (NBP3-45827) is a polyclonal antibody validated for use in IHC, WB, ELISA, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: small proline-rich protein 3. (Uniprot# Q9UBC9)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPRR3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Immunoprecipitation 1:200-1:1000
  • Western Blot 1:500-1:2000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SPRR3 Antibody - BSA Free

• 22 kDa pancornulin
• Cornifin beta
• esophagin
• small proline-rich protein 3
• SPRC

Background

SPRR3, also known as Small proline-rich protein 3, consists of a 169 amino acid isoform that is 18 kDa, and is involved in the surrounding and protection of keratinocytes. Disease research on the protein is currently being conducted with ichthyosis vulgaris, psoriasis, eosinophilic esophagitis, verrucous carcinoma, periodontitis, esophageal cancer, eczema, tonsillitis, and skin disease. SPRR3 is linked to several biological processes, including epidermis development, wound healing, and keratinocyte differentiation, in which it interacts with other proteins, such as IVL, EVPL, NFKBIA, and LOR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPRR3