SPRED2 Antibody [Alexa Fluor® 488]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

SPRED2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human SPRED2
  Lys117-Ser297
  Accession # Q7Z698
• Specificity: Detects human SPRED2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SPRED1 is observed and less than 3% cross-reactivity with rhSPRED4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SPRED2 (Sprouty-related protein with an EVH1 domain-2) is a 47-53 kDa member of the SPRED family of regulatory molecules. In human, it is found in keratinocytes, intestinal columnar epithelium, and salivary duct epithelium. It inhibits receptor tyrosine kinase (RTK) signaling by blocking Ras activation of Raf. This is achieved by inducing the lysosomal degradation of RTKs following SPRED2 binding to NBR1. Human SPRED2 is 418 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 5-122), a central KBD domain that binds to the SCFR (aa 201-257), and a C-terminal Sprouty‑related domain that mediates homodimerization, and heterodimerization with SPRED1 (aa 308-416). SPRED is ubiquitinated, and undergoes phosphorylation on Ser, Thr and Tyr residues. There is one splice variant that shows a six aa substitution for aa 1-9. Over aa 117-297, human SPRED2 shares 84% aa identity with mouse SPRED2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.