SPR Antibody

Images

 
Western Blot: SPR Antibody [NBP1-33546] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: SPR Antibody [NBP1-33546] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SPR Antibody [NBP1-33546] - Paraffin-embedded mouse fore brain. SPR antibody [N2C3] dilution: 1:500.
Immunoprecipitation: SPR Antibody [NBP1-33546] - A431 whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of SPR protein by 4 ug SPR antibody [N2C3] 5 % SDS-PAGE The ...read more

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SPR Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SPR. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Monkey (97%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SPR
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (97%), Bovine (82%), Rabbit (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPR Antibody

  • EC 1.1.1.153
  • SDR38C1
  • sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase)
  • sepiapterin reductase
  • short chain dehydrogenase/reductase family 38C, member 1

Background

This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SPR Antibody (NBP1-33546) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SPR