Recombinant Human SPINK1 Protein

Product Discontinued

Recombinant Human SPINK1 Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 24-79 of Human SPINK1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSDSLGREA KCYNELNGCT KIYDPVCGTD GNTYPNECVL CFENRKRQTS ILIQKSGPC

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SPINK1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 8.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human SPINK1 Protein

• pancreatic secretory trypsin inhibitor
• PCTT
• PCTTSpink3
• PSTI
• PSTISerine protease inhibitor Kazal-type 1
• serine peptidase inhibitor, Kazal type 1
• serine protease inhibitor, Kazal type 1
• SPINK1
• Spink3
• TATI
• TATITumor-associated trypsin inhibitor

Background

SPINK1 is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. Recombinant human SPINK1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SPINK1