SPINK1 Antibody (839305) [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB
• Clone: 839305
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 700

SPINK1 Antibody (839305) [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human SPINK1
  Asp24-Cys79
  Accession # P00995
• Specificity: Detects human SPINK1 in ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SPINK1 Antibody (839305) [Alexa Fluor® 700]

• pancreatic secretory trypsin inhibitor
• PCTT
• PCTTSpink3
• PSTI
• PSTISerine protease inhibitor Kazal-type 1
• serine peptidase inhibitor, Kazal type 1
• serine protease inhibitor, Kazal type 1
• SPINK1
• Spink3
• TATI
• TATITumor-associated trypsin inhibitor

Background

SPINK1 (Serine Protease Inhibitor Kazal-type 1; also TATI and PST1) is a 6-7 kDa secreted polypeptide initially identified as a tumor-derived trypsin inhibitor. It is widely expressed and found in cells diverse as pancreatic acinar cells, columnar cells of the stomach, renal collecting duct epithelium, and ureteric transitional plus breast epithelium. SPINK1 is known to be secreted with pancreatic zymogens, and apparently inactivates prematurely-activated trypsin, thus protecting the pancreas from trypsin-mediated enzyme activation. It also is reported to regulate cell migration and proliferation, the latter effect attributed to its structural resemblance to EGF and its ability to bind to activate the EGFR. Mature human SPINK1 is 56 amino acids (aa) in length (aa 24-79). It contains one Kazal-like domain (aa 26-79) that possesses a potential proteolytic cleavage site between Lys41-Ile42. An 11-12 kDa form in SDS-PAGE has been reported for SPINK1, possibly reflecting dimerization. Mature human SPINK1 shares 66% aa sequence identity with mouse Spink3, the mouse equivalent to human SPINK1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.