Spectrin alpha 1 Antibody (SB-SP1)

Western Blot: Spectrin alpha 1 Antibody (SB-SP1) [NB100-2070] - Erythrocytes ghost extract was separated on SDS-PAGE and probed with Monoclonal AB To Spectrin (MO) CL:SB-SP1 (Prepack). The antibody was developed with HRP APA Mouse Fab ads/HIgG and AEC substrate. Antibody dilution 1/1,000.

Product Discontinued

Spectrin alpha 1 Antibody (SB-SP1) Summary

• Immunogen: Full length native Human erythroid spectrin was used as the immunogen (purified).
• Localization: Cytoplasmic surface of the cell membrane
• Specificity: Localizes the 220 kDa (beta) and 240 kDa (alpha) bands of human erythrocyte spectrin staining the beta subunit stronger than the alpha subunit at the recommended titer. In addition, a number of polypeptides in the region of 170, 150, 85, and 45 kDa will react with the antibody. It is likely these lower molecular weight polypeptides may have an epitope shared by both the alpha and beta subunits of spectrin. By immunofluorescence the antibody will stain human spectrin in erythrocyte smears.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPTA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Raw ascites
• Preservative: 0.09% Sodium Azide
• Purity: Unpurified

Alternate Names for Spectrin alpha 1 Antibody (SB-SP1)

• alpha-I spectrin
• EL2
• erythrocyte
• HS3
• spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Background

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Spectrin alpha 1 Antibody (NB100-2070)(1)

Publication using NB100-2070

• Transforming Growth Factor-[beta]1 Antagonizes Interleukin-5 Pro-Survival Signaling by Activating Calpain-1 in Primary Human Eosinophils. Xie Q, She Z-J, Oh J et al. J Clin Cell Immunol [PMID: 24244891] (WB, Human)

Bioinformatics

• Gene Symbol: SPTA1