Spectrin alpha 1 Antibody (AF10)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-Fr, IP
Clone
AF10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.1 mg/ml

Order Details

Spectrin alpha 1 Antibody (AF10) Summary

Immunogen
The antibody reacts with the Erythroid alpha-Spectrin antigen.
Specificity
The antibody is specific to the 240kD erythroid a-spectrin.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SPTA1
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Various immunochemical reactions to detect erythroid spectrins. Identification of erythroid leukemias (1). Works in immunoprecipitation, western blotting and immunocytochemistry on frozen sections.Working dilution in western blotting is at least 1:1000.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS solution containing 1.0% w/v BSA
Preservative
0.1% Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Alternate Names for Spectrin alpha 1 Antibody (AF10)

  • alpha-I spectrin
  • EL2
  • erythrocyte
  • HS3
  • spectrin, alpha, erythrocytic 1 (elliptocytosis 2)

Background

Spectrin is a major constituent of the erythrocyte skeleton accounting for 25% of the total membrane protein and 75% of the cytoskeletal mass. It is associated with the cytoplasmic surface of the membrane by attachment to ankyrin, a peripheral membrane protein. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface and transmembrane movement of phospholipids and cholesterol. On SDS PAGE gels erythrocyte spectrin appears as two bands referred to as alpha (240 kDa) and beta (220 kDa). While the simplest form of spectrin in solution is an alpha-beta heterodimer, spectrin can undergo self-association in various conditions to form a tetramer of alpha2-beta2. In recent years a large number of spectrin-like molecules have been found in non-erythroid cells. These proteins are known by such different names as fodrin, CBP I, calspectin and TW 260/240.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SPTA1
Entrez
Uniprot