Human SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric)

ELISA: Human SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) [NBP2-76695] - Samples were spiked with high concentrations of Human SPARC-like 1/SPARCL1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) [NBP2-76695] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL

Human SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 0.313 - 20ng/mL
• Sensitivity: 0.188ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.46
• Intra-Assay: CV% < 5.3
• Spike Recovery: 88-109%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SPARCL1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human SPARC-like 1/SPARCL1 ELISA Kit (Colorimetric)

• Hevin
• High endothelial venule protein
• MAST 9
• MAST9
• PIG33
• proliferation-inducing protein 33
• SC1
• SPARC like 1
• SPARCL1
• SPARC-like 1 (hevin)
• SPARC-like 1 (mast9, hevin)
• SPARC-like 1
• SPARC-like protein 1

Background

Human SPARC-like 1 (SPARCL1), also known as MAST9 or Hevin, is a member of the SPARC protein family. It is a secreted acidic calcium-binding protein of 664 amino acids that shows 62% identity with SPARC. SPARCL1 is a secreted protein, found in the extracellular matrix. It is expressed at high levels in high endothelial venules (HEV) in lymphoid tissues. SPARCL1 inactivation is a common event in tumors of epithelial origin and levels are down-regulated in a variety of tumors. It may have tumor suppressor activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for SPARC-like 1/SPARCL1 ELISA Kit (NBP2-76695)(1)

Publication using NBP2-76695

• Acharya S, Prabhu P, Patil VS et al. Immunohistochemical expression of tumor necrosis factor-like weak inducer of apoptosis and fibroblast growth factor-inducible immediate early response protein 14 in oral squamous cell carcinoma and its implications J Investig Clin Dent 2019-09-21 [PMID: 31541512]

Bioinformatics

• Gene Symbol: SPARCL1