SPAG6 Antibody

Western Blot: SPAG6 Antibody [NBP2-20462] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: SPAG6 Antibody [NBP2-20462] - Immunohistochemical analysis of paraffin-embedded H441 xenograft, using antibody at 1:500 dilution.

Product Discontinued

SPAG6 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human SPAG6. The exact sequence is proprietary.
• Localization: Cytoplasm; cytoskeleton; Cell projection; cilium; flagellum
• Predicted Species: Mouse (92%), Rat (95%), Porcine (95%), Bovine (95%), Primate (98%), Xenopus (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPAG6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (98%), Xenopus laevis (92%), Zebrafish (87%), Chicken (89%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for SPAG6 Antibody

• axoneme central apparatus protein
• DKFZp434I153
• pf16
• Protein PF16 homolog
• Repro-SA-1MGC26276
• sperm associated antigen 6
• Sperm flagellar protein
• sperm-associated antigen 6

Background

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats, a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SPAG6
• Uniprot:
  • O75602(Human)