SP17 Overexpression Lysate (Native)

Images

 
Western Blot: SP17 Overexpression Lysate (Adult Normal) [NBL1-16361] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SP17.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

SP17 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207076 and based on accession number NM_017425. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens sperm autoantigenic protein 17 (SPA17), mRNA.
Gene
SPA17

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for SP17

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for SP17 Overexpression Lysate (Native)

  • Cancer/testis antigen 22
  • CT22SP17-1
  • Sp17-1
  • SP17sperm surface protein Sp17
  • sperm autoantigenic protein 17Sperm protein 17

Background

This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for SP17 Lysate (NBL1-16361) (0)

There are no publications for SP17 Lysate (NBL1-16361).
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Video Protocols

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Bioinformatics

Gene Symbol SPA17