Rat SP-B/Surfactant Protein B ELISA Kit (Colorimetric)

ELISA: Rat SP-B/Surfactant Protein B ELISA Kit (Colorimetric) [NBP2-76691] - Samples were spiked with high concentrations of Rat SP-B/Surfactant Protein B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat SP-B/Surfactant Protein B ELISA Kit (Colorimetric) [NBP2-76691] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.563 - 100ng/mL
• Sensitivity: 0.938ng/mL

Rat SP-B/Surfactant Protein B ELISA Kit (Colorimetric) Summary

• Standard Curve Range: 1.563 - 100ng/mL
• Sensitivity: 0.938ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.89
• Intra-Assay: CV% < 5.02
• Spike Recovery: 85-103%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: SFTPB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat SP-B/Surfactant Protein B ELISA Kit (Colorimetric)

• 18 kDa pulmonary-surfactant protein
• 6 kDa protein
• PSP-B
• pulmonary surfactant-associated protein B
• Pulmonary surfactant-associated proteolipid SPL(Phe)
• SFTB3
• SFTP3
• SFTPB
• SMDP1
• SP-B surfactant, pulmonary-associated protein B
• SP-B
• surfactant protein B

Background

The SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for SP-B/Surfactant Protein B ELISA Kit (NBP2-76691). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: SFTPB