SP-B/Surfactant Protein B Antibody

Product Discontinued

SP-B/Surfactant Protein B Antibody Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Secreted
• Specificity: Reacts strongly with human and mouse proSP B (detects both the N-terminal and C-terminal propeptides). Does not react with mature SP B. On human tissue immunostains alveolar Type II cells and non-ciliated bronchiolar cells. On mouse tissue immunostains alveolar Type II cells and non-ciliated bronchiolar (Clara cells) in distal and terminal airways.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFTPB
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:250-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2500
• Application Notes: This antibody can be used in ELISA, Immunohistochemistry-Paraffin, and Western Blot. Optimal dilutions should be determined by the end user.

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera with 40% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: Unpurified

Alternate Names for SP-B/Surfactant Protein B Antibody

• 18 kDa pulmonary-surfactant protein
• 6 kDa protein
• PSP-B
• pulmonary surfactant-associated protein B
• Pulmonary surfactant-associated proteolipid SPL(Phe)
• SFTB3
• SFTP3
• SFTPB
• SMDP1
• SP-B surfactant, pulmonary-associated protein B
• SP-B
• surfactant protein B

Background

The SFTPB gene encodes the pulmonary-associated surfactant B protein (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a lipid-rich material that prevents lung collapse by lowering surface tension at the air-liquid interface in the alveoli of lung. SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Surfactant is composed of phospholipids and other surfactant-associated proteins (Clark et al., 1995 [PubMed 7644495]). See also SFTPA1 (MIM 178630), SFTPC (MIM 178620), and SFTPD (MIM 178635).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SP-B/Surfactant Protein B Antibody (NB600-1115)(1)

Publication using NB600-1115

• Guo H, Kazadaeva Y, Ortega FE et al. Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung Dev. Biol. 2017-08-12 [PMID: 28811219] (WB, Mouse)

Bioinformatics

• Gene Symbol: SFTPB
• Entrez:
  • 6439(Human)
  • 20388(Mouse)