SOST/Sclerostin Antibody (220902) [Unconjugated]

Response of serum sclerostin and Wnt/ beta ‐catenin signaling within scWAT to 4 weeks of SIT. (a) Serum sclerostin response to SIT. (b) Representative immunoblots of sclerostin, phosphorylated and total GSK3 beta , beta ‐catenin, and total protein loading control (Ponceau S) at pre‐SIT (labeled “a”) and post‐SIT (labeled “b”) with estimated molecular weights on the right. These representative samples are the same two participants (“1” and “2”) in sequence across proteins that highlight their response from pre‐ to post‐SIT. scWAT (c) sclerostin content, (d) total and phosphorylated GSK3 beta , and (e) total beta ‐catenin response to SIT relative to pre‐SIT. Bar charts have black lines that represent individual responses to SIT with group means presented as white (pre‐SIT) and gray (post‐SIT) bars ± SD. Paired t‐tests were used to examine changes pre‐ to post‐SIT Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35312183), licensed under a CC-BY license. Not internally tested by R&D Systems.

Response of eWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Response of eWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Response of iWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

Response of iWAT sclerostin content (A), serine9 phosphorylation status (B,D) both total GSK3b content (C), and total b-catenin content (E) to HFD and acute exercise, as well as representative immunoblots (F). Light grey bars represent sedentary mice and dark grey bars represent mice sampled 2 h following 2 h of acute endurance exercise and are grouped by diet; LFD = low fat diet; HFD = high fat diet. Proteins are corrected for by the house keeping protein vinculin and are all relative to LFD sedentary mice, data are presented as means ± SD and main effects are presented on graphs only if they are either close to or are significant. A two-way factorial ANOVA was used to examine main effects for diet and exercise as well their interaction. Tukey correction was used for multiple pairwise comparisons. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36685192), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, ELISA(Cap)
• Clone: 220902
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

SOST/Sclerostin Antibody (220902) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human SOST/Sclerostin
  Gln24-Tyr213
  Accession # Q9BQB4
• Specificity: Detects human SOST/Sclerostin in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse SOST is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SOST
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Capture: Human SOST/Sclerostin Antibody (Catalog # MAB1406)
  ELISA Detection: Human SOST/Sclerostin Biotinylated Antibody (Catalog # BAM14061)
  Standard: Recombinant Human SOST/Sclerostin (Catalog # 1406-ST)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOST/Sclerostin Antibody (220902) [Unconjugated]

• sclerostin
• SOST
• VBCHsclerosteosis

Background

SOST, also known as sclerostin, belongs to the Cerberus/DAN family of secreted glycoproteins. SOST binds BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity and functions in bone homeostasis as a BMP antagonist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SOST/Sclerostin Antibody (MAB1406)(14)

Publications using MAB1406

• N Kurgan, B Baranowski, J Stoikos, AJ MacNeil, VA Fajardo, REK MacPherson, P Klentrou Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male mice Frontiers in Physiology, 2023-01-04;13(0):1061715. 2023-01-04 [PMID: 36685192] (Western Blot, Mouse)
• VK Kuna, A Lundgren, LO Anerillas, P Kelk, M Brohlin, M Wiberg, PJ Kingham, LN Novikova, G Andersson, LN Novikov Efficacy of Nerve-Derived Hydrogels to Promote Axon Regeneration Is Influenced by the Method of Tissue Decellularization International Journal of Molecular Sciences, 2022-08-06;23(15):. 8/6/2022 [PMID: 35955880]
• N Kurgan, H Islam, JBL Matusiak, BJ Baranowski, J Stoikos, VA Fajardo, REK MacPherson, BJ Gurd, P Klentrou Subcutaneous adipose tissue sclerostin is reduced and Wnt signaling is enhanced following 4-weeks of sprint interval training in young men with obesity Physiological Reports, 2022-03-01;10(6):e15232. 3/1/2022 [PMID: 35312183]
• Qiushi Wei, Bin Wang, Hailan Hu, Chuhai Xie, Long Ling, Jianliang Gao et al. Icaritin promotes the osteogenesis of bone marrow mesenchymal stem cells via the regulation of sclerostin expression International Journal of Molecular Medicine 2020-01-20 [PMID: 31985018] (Western Blot, Immunocytochemistry, Human)
• CJM Stapledon, H Tsangari, LB Solomon, DG Campbell, P Hurtado, R Krishnan, GJ Atkins Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis PLoS ONE, 2019-09-26;14(9):e0222602. 2019-09-26 [PMID: 31557169] (ICC, Human)
• Yusuke Inagaki, Edward S Hookway, Takeshi G Kashima, Mitsuru Munemoto, Yasuhito Tanaka, Andrew Bassim Hassan et al. Sclerostin expression in bone tumours and tumour-like lesions Histopathology 2016-09-01 [PMID: 26896083]
• Pereira R, Valta H, Tumber N, Salusky I, Jalanko H, Makitie O, Wesseling Perry K Altered Osteocyte-Specific Protein Expression in Bone after Childhood Solid Organ Transplantation. PLoS ONE, 2015-09-21;10(9):e0138156. 2015-09-21 [PMID: 26390291] (IHC, Human)
• Agnetha Folestad, Martin Ålund, Susanne Asteberg, Jesper Fowelin, Ylva Aurell, Jan Göthlin et al. Role of Wnt/ beta -catenin and RANKL/OPG in bone healing of diabetic Charcot arthropathy patients Acta Orthopaedica 2015-07-21 [PMID: 25811776]
• Pereira R, Juppner H, Gales B, Salusky I, Wesseling-Perry K Osteocytic protein expression response to doxercalciferol therapy in pediatric dialysis patients. PLoS ONE, 2015-03-16;10(3):e0120856. 2015-03-16 [PMID: 25774916] (IHC, Human)
• Daniel Cejka, Johann Herberth, Adam J. Branscum, David W. Fardo, Marie-Claude Monier-Faugere, Danielle Diarra et al. Sclerostin and Dickkopf-1 in Renal Osteodystrophy Clinical Journal of the American Society of Nephrology 2011-04-01 [PMID: 21164019]
• Jilka RL, O'Brien CA, Bartell SM, Weinstein RS, Manolagas SC Continuous elevation of PTH increases the number of osteoblasts via both osteoclast-dependent and -independent mechanisms. J. Bone Miner. Res., 2010-11-01;25(11):2427-37. 2010-11-01 [PMID: 20533302] (Western Blot, Mouse)
• Modder UI, Clowes JA, Hoey K, Peterson JM, McCready L, Oursler MJ, Riggs BL, Khosla S Regulation of circulating sclerostin levels by sex steroids in women and in men. J. Bone Miner. Res., 2011-01-01;26(1):27-34. 2011-01-01 [PMID: 20499362] (ELISA Development, Human)
• Appel H, Ruiz-Heiland G, Listing J, Zwerina J, Herrmann M, Mueller R, Haibel H, Baraliakos X, Hempfing A, Rudwaleit M, Sieper J, Schett G Altered skeletal expression of sclerostin and its link to radiographic progression in ankylosing spondylitis. Arthritis Rheum., 2009-11-01;60(11):3257-62. 2009-11-01 [PMID: 19877044] (ELISA Development, Human)
• Jager A, Gotz W, Lossdorfer S, Rath-Deschner B Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro. J. Periodont. Res., 2009-09-23;45(2):246-54. 2009-09-23 [PMID: 19778325] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: SOST
• Uniprot:
  • Q9BQB4()