Somatostatin R1/SSTR1 Antibody

Product Discontinued

Somatostatin R1/SSTR1 Antibody Summary

• Immunogen: A synthetic peptide derived from the C-terminus of rat somatostatin receptor protein.
• Localization: Cytoplasmic
• Specificity: This reacts with a 60 kD protein, known as Somatostatin. Receptor.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SSTR1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: IHC: This antibody may be diluted to a titer of 1:50-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Use in Immunocytochemistry/Immunofluorescence was reported in a scientificd publication (PMID: 23691031).

Reactivity Notes

Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS with BSA
• Preservative: Sodium Azide
• Purity: IgG purified

Notes

Licensed to Novus Biologicals LLC under U.S. Patent No 5,998,154.

Alternate Names for Somatostatin R1/SSTR1 Antibody

• G-protein coupled receptor
• Somatostatin R1
• somatostatin receptor 1
• somatostatin receptor type 1
• SomatostatinR1
• SRIF-2
• SS1R
• SS-1-R
• SS1-R
• SSR1
• SSTR1

Background

The Somatostatin family of receptors have five members, sst1-sst5, as well as two isoforms of Somatostatin receptor 2, sst2A and sst2B. They are coupled to G protein and are involved in regulation of numerous processes including secretion of insulin, glucagons and growth hormone and excitation of neurons in central and peripheral nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Somatostatin R1/SSTR1 Antibody (NB120-2366)(1)

Publication using NB120-2366

• Moller TC, Wirth VF, Roberts NI et al. PDZ domain-mediated interactions of g protein-coupled receptors with postsynaptic density protein 95: quantitative characterization of interactions. PLoS One 2013-05-14 [PMID: 23691031] (ICC/IF, Mouse)

FAQs for Somatostatin R1/SSTR1 Antibody (NB120-2366). (Showing 1 - 1 of 1 FAQ).

1. We are interested in using anti-SSTR1 for an experiment where we need the antibody to bind to receptors in living cells. Is the epitope on SSTR1 in the extracellular part of the receptor (N-terminus or extracellular loops)?
   • The SSTR1 receptor immunogen maps to the C-terminal region of rat somatostatin protein. The localization is cytoplamic and we recommend pancreas as a positive control tissue.

Bioinformatics

• Gene Symbol: SSTR1
• Entrez:
  • 20605(Mouse)
  • 25033(Rat)