Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

Western Blot: Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) [NBP3-32062] - Western blot analysis of Sodium Potassium ATPase Alpha 3 on different lysates with Rabbit anti-Sodium Potassium ATPase Alpha 3 antibody (NBP3-32062) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Mouse brain tissue lysate (70 heat) Lane 3: Mouse brain tissue lysate (no heat) Lane 4: Rat brain tissue lysate Lane 5: Rat brain tissue lysate (no heat) Lane 6: Mouse cerebellum tissue lysate Lane 7: Mouse cerebellum tissue lysate (70 heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 112 kDa Observed band size: 100/150 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32062) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: JE35-24
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) Summary

• Description: Novus Biologicals Rabbit Sodium Potassium ATPase Alpha 3 Antibody (JE35-24) (NBP3-32062) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human Sodium Potassium ATPase Alpha 3 aa 1-50 / 1,013. (Uniprot: P13637)
• Localization: Cell membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ATP1A3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Sodium Potassium ATPase Alpha 3 Antibody (JE35-24)

• alpha 3 polypeptide
• ATP1A3
• ATPase, Na+/K+ transporting, alpha 3 polypeptide
• dystonia 12
• DYT12
• EC 3.6.3
• MGC13276
• RDP
• Sodium Potassium ATPase Alpha 3
• Sodium pump subunit alpha-3
• sodium/potassium-transporting ATPase alpha-3 chain
• sodium/potassium-transporting ATPase subunit alpha-3

Background

The sodium/potassium ATPase is an integral membrane enzyme found in all cells of higher organisms and is responsible for the ATP-dependent transport of sodium and potassium across the cell membrane. This membrane-bound enzyme is related to a number of other ATPases including sarcoplasmic and endoplasmic reticulum calcium ATPase (SERCA) and plasma membrane calcium ATPase (PMCA). The sodium/potassium ATPase consists of a large, multipass, transmembrane catalytic subunit, termed the alpha subunit, and an associated smaller glycoprotein, termed the beta subunit. Studies indicate that there are three isoforms of the alpha subunit (alpha 1, alpha 2, alpha 3) and two isoforms of the beta subunit (beta 1 and beta 2) encoded by two multigene families. The different isoforms of the sodium/potassium ATPase exhibit tissue-specific and developmental patterns of expression. The alpha 1 and beta mRNAs are present in all cell types examined, whereas the alpha 2 and alpha 3 mRNAs exhibit a more restricted pattern of cell-specific expression. The alpha-3 subunit has been found in neuronal and to skeletal and cardiac muscle, lung and stomach tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATP1A3