SNX15 Antibody

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Western Blot: SNX15 Antibody [NB100-1210] - (0.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SNX15 Antibody Summary

Immunogen
Peptide with sequence C-EEILRLHLSQLPP corresponding to C-Terminus according to NP_037438.2, NP_680086.2.
Specificity
This antibody is expected to recognise both Human isoforms according to NP_037438.2 and NP_680086.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SNX15
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Approx. 48 kDa band observed in human kidney lysates and in HEK293 lysates (calculated MW 38.2 kDa band according to NP_037438.2). Note: A single approx. 30-35 kDa band (probably representing the shorter isoform) was observed in human heart.
Positive Control
293 Lysate (NB800-PC6)
Kidney Lysate (NB820-59231)
Publications
Read Publication using NB100-1210.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SNX15 Antibody

  • clone iota unknown protein
  • HSAF001435
  • sorting nexin 15
  • sorting nexin-15

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. This gene encodes two transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SNX15 Antibody (NB100-1210)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SNX15 Antibody (NB100-1210) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SNX15
Entrez
Uniprot