SNX15 Antibody

Western Blot: SNX15 Antibody [NB100-1210] - (0.5ug/ml) of 293 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

SNX15 Antibody Summary

• Description: Novus Biologicals Goat SNX15 Antibody (NB100-1210) is a polyclonal antibody validated for use in WB and ELISA. Anti-SNX15 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EEILRLHLSQLPP corresponding to C-Terminus according to NP_037438.2, NP_680086.2.
• Specificity: This antibody is expected to recognise both Human isoforms according to NP_037438.2 and NP_680086.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SNX15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 1:1000
• Application Notes: WB: Approx. 48 kDa band observed in human kidney lysates and in HEK293 lysates (calculated MW 38.2 kDa band according to NP_037438.2). Note: A single approx. 30-35 kDa band (probably representing the shorter isoform) was observed in human heart.
• Control:

  293 Whole Cell Lysate
  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SNX15 Antibody

• clone iota unknown protein
• HSAF001435
• sorting nexin 15
• sorting nexin-15

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. This gene encodes two transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SNX15 Antibody (NB100-1210)(1)

Publication using NB100-1210

• Phillips SA, Barr VA, Haft DH et al. Identification and characterization of SNX15, a novel sorting nexin involved in protein trafficking. J Biol Chem 2001-02-16 [PMID: 11085978]

Bioinformatics

• Gene Symbol: SNX15
• Entrez:
  • 29907(Human)
• Uniprot:
  • NP_037438(Human)