SNW1 Antibody - BSA Free

Western Blot: SNW1 Antibody [NBP1-77304] - Analysis of SkiP in mouse skeletal muscle tissue lysate with SkiP antibody at 0.5 ug/ml.

Immunohistochemistry: SNW1 Antibody - BSA Free [NBP1-77304] - Immunofluorescence of Ski in human kidney tissue with Ski antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SNW1 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human SkiP. The immunogen is located within the last 50 amino acids of SkiP. Amino Acid Squence: SRPKEHEHEGKKRRKE
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNW1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 20 ug/ml
  • Western Blot 0.5 ug/ml
• Theoretical MW: 65 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SNW1 Peptide (NBP1-77304PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SNW1 Antibody - BSA Free

• Bx42
• homolog of Drosophila BX42
• MGC119379
• NCoA-62
• Nuclear protein SkiP
• Nuclear receptor coactivator NCoA-62
• nuclear receptor coactivator, 62-kD
• Prp45
• PRPF45
• SKI interacting protein
• Ski-interacting protein
• SKIPSKIIP
• SNW domain containing 1
• SNW domain-containing protein 1

Background

TGF-beta and the bone morphogenic proteins (BMPs) are key signaling proteins that regulate numerous cellular processes such as embryonic development and tumorigenesis. Both signal through the Smad protein family and are negatively regulated by Ski and SnoN, two related proto-oncoproteins. Ski functions by binding to the Smad proteins activated by TGF-beta and the (BMPs) and preventing their phosphorylation, inhibiting their ability to bind DNA and activate the transcription of downstream genes. SkiP was originally identified as a Ski-interacting protein and was later found to augment the signals induced by TGF-beta but inhibit transcription induced by BMP-2 in C2C12 cells, suggesting that SkiP is a key player in the signaling cascades inititated by TGF-beta and the BMP protein family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SNW1
• Uniprot:
  • NP_036377(Human)