SNF1LK2/SIK2 Knockout HeLa Cell Lysate

Product Discontinued

SNF1LK2/SIK2 Knockout HeLa Cell Lysate Summary

• Preparation Method: Knockout achieved by using CRISPR/Cas9,Insertion of the selection cassette in exon 6
• Gene: SIK2

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines.
• Concentration: LYOPH
• Reconstitution Instructions: To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel.

Lysate Details for Array

• Type: Knockout HeLa Cell

Notes

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Validation of antibody specificity is critical and verification of antibody performance against knockout samples is one way to guarantee that an antibody recognizes a specific target. Novus' KO cell lysate can be used as a negative control for western blots and to confirm the specificity of antibodies.

Alternate Names for SNF1LK2/SIK2 Knockout HeLa Cell Lysate

• DKFZp434K1115
• EC 2.7.11
• EC 2.7.11.1
• KIAA0781SIK-2
• LOH11CR1I
• QIK
• QIKSNF1LK2
• Qin-induced kinase
• salt-inducible kinase 2
• Salt-inducible protein kinase 2
• salt-inducible serine/threonine kinase 2
• serine/threonine-protein kinase SIK2
• Serine/threonine-protein kinase SNF1-like kinase 2
• SIK2
• SNF1-like kinase 2
• SNF1LK2

Background

The protein Salt Inducible Kinase 2 (SIK2) has been found to play a critical role in cell division and regulating the response of ovarian cancer to chemotherapy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: SIK2