SNAP23 Antibody [Alexa Fluor® 405]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

SNAP23 Antibody [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human SNAP23
  Asp146-Ser211
  Accession # O00161
• Specificity: Detects human SNAP23 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse SNAP23 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

SNAP23 (Synaptosomal associated protein 23; also Syndet) is a membrane-associated 23‑29 kDa member of the SNAP25 family of proteins. It is expressed in multiple cell types, including neutrophils, platelets, mast cells and adipocytes. SNAP23 is involved in vesicle exocytosis. It has been proposed that SNAP23 can associate with both vesicle and target (cell) membranes via a lipid modification. As a v-SNARE, it may interact with syntaxin-6 at the cell membrane. As a t-SNARE, in conjunction with syntaxin-4, it likely interacts with VAMP-2 and -8 on the vesicle membrane. In either case, this approximates two membranes, which subsequently fuse to create a pore. Human SNAP23 is 211 amino acids (aa) in length. It contains two t-SNARE coiled-coil homology domains (aa 14‑76 and 148‑207). Palmitoylation occurs between aa 80‑87, while phosphorylation occurs on multiple Ser/Thr residues. There is one splice variant that shows a deletion of aa 90‑142. Over aa 146‑211, human and mouse SNAP23 share 85% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.