SMYD5 Antibody

Immunohistochemistry-Paraffin: SMYD5 Antibody [NBP1-31222] - Paraffin-embedded mouse ovary. SMYD5 antibody [C2C3], C-term diluted at 1:500.

Immunohistochemistry-Paraffin: SMYD5 Antibody [NBP1-31222] - Paraffin-embedded NCI-N87 xenograft, using antibody at 1:100 dilution.

Western Blot: SMYD5 Antibody [NBP1-31222] -Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD5 antibody [C2C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: SMYD5 Antibody [NBP1-31222] - Sample (30 ug of whole cell lysate)A: U87-MG10% SDS PAGE diluted at 1:1000

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SMYD5 Antibody Summary

• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SMYD5. The exact sequence is proprietary.
• Predicted Species: Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMYD5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SMYD5 Antibody

• NN8-4AG
• Protein NN8-4AG
• RAI15
• retinoic acid induced 15
• retinoic acid responsive
• Retinoic acid-induced protein 15
• RRG1
• SET and MYND domain-containing protein 5
• SMYD family member 5
• ZMYND23

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SMYD5 Antibody (NBP1-31222)(1)

Publication using NBP1-31222

• Hou Y, Sun X, Gheinani PT et al. Epithelial SMYD5 Exaggerates IBD by Downregulating Mitochondrial Functions via Post-translational Control of PGC-1 alpha Stability Cellular and molecular gastroenterology and hepatology 2022-05-25 [PMID: 35643234] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: SMYD5
• Entrez:
  • 10322(Human)
• Uniprot:
  • Q6GMV2(Human)